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BARBITURATES (BAR)

QuickScreen™ One-Step Rapid Drug Screen Test (Cassette type)

A Professional, Forensic Diagnostic Test for use in the Qualitative Detection of Barbiturates and their metabolites in Human Urine at a Sensitivity Level of 300 ng/ml Secobarbital Established as Standard by the U.S. Department of Health and Human Services, DOT, World Health Organization and SAMHSA.

INTENDED USE

For a comprehensive discussion on drug of abuse testing including typical detection time periods see the DRUG TESTING FAQ

The QuickScreen™ One-Step Rapid Barbiturates Test is a rapid, visual, qualitative, competitive binding immunoassay for the detection of Barbiturates in human urine. The test provides a fast confirmation of the presence of Barbiturates in the subject sample at a minimum cutoff sensitivity level of 300 ng/ml BAR, the sensitivity level set by the National Institute on Drug Abuse and Departments of Health and Human Services. Note: Where indicated, any positive test should be confirmed using quantitative methods by a qualified testing laboratory.
Note: This assay provides only a preliminary analytical test result. A more specific alternative chemical method such as high-pressure liquid chromatography (HPLC) or Gas chromatography/mass spectrometry (GC/MS) must be used in order to obtain a confirmed analytical result. For test situations requiring laboratory confirmation by such methods please refer to the QuickScreen Pro At Home Test Kit. Clinical consideration and professional judgment should be applied to all test results, particularly when preliminary positive results are indicated.

SUMMARY AND EXPLANATION OF THE TEST

Barbiturates are a class of prescription drugs frequently abused. Barbiturates in normally prescribed doses act in depressing the central nervous system while overdoses induce exhilaration, sedation, and respiratory depression. Acute overdose results in respiratory collapse, coma, and if not treated promptly, death. Classified generally as depressants, barbiturates produce a state of intoxication that is remarkably similar to alcohol intoxication. Symptoms include slurred speech, loss of motor coordination and impaired judgment. Depending on the dose, frequency, and duration of use, one can rapidly develop tolerance, physical dependence and psychological dependence on barbiturates.  Barbiturate abusers prefer the short-acting and intermediate-acting barbiturates pentobarbital (Nembutal),  secobarbital (Seconal) and amobarbital (Amytal). Other short-and intermediate-acting barbiturates are butalbital (Fiorinal, Fioricet), butabarbital (Butisol), talbutal (Lotusate) and aprobarbital (Alurate). After oral administration, the onset of drug effect is from 15 to 40 minutes and the effects last up to 6 hours depending on dose administered and body type. Teenagers often abuse barbiturates for the temporary "high" achieved that simulates alcohol intoxication. For comprehensive information on drug testing including typical half-life or drug detection periods see the Drug Testing FAQ.

The QuickScreen™ One-Step Rapid Barbiturates Test utilizes a unique combination of monoclonal antibodies to selectively identify Barbiturates and their metabolites in urine with a high degree of sensitivity. The test kit is a rapid, visually read screening method that does not require special instrumentation or training.

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Drug Test ResultThe QuickScreen™ One-Step Rapid Barbiturates Test technology incorporates a chromatographic absorbent device in which the drug or drug metabolites in the sample compete with a Barbiturates derivative immobilized on a porous membrane for limited antibody sites. This is the preferred scientific method for qualitative assay. In the assay procedure, urine mixes with labeled antibody-dye conjugate and migrates through test device. When Barbiturates levels are below 300 ng/ml (the detection cutoff sensitivity of the test) unbound antibody-dye conjugate binds to immobilized antigen conjugate in the Test Zone ("T"), producing a pink-rose colored band that indicates a negative result. Conversely, when Barbiturates levels are above the detection limit, antibody-dye conjugate binds to the free drug, forming an antigen-antibody-dye complex. The complex competes with immobilized antigen conjugate in the Test Zone, preventing the development of a pink-rose colored band. Regardless of the test result, a color band is produced in the Control Zone ("C") by a non-specific sandwich dye conjugate reaction. This band serves as a built-in quality control by demonstrating antibody recognition, verifying that the reagents are chemically active, and confirming that the test was performed correctly.

MATERIALS PROVIDED:

Test Device and Dropper Pipette individually sealed in foil pouch with complete instructions.

ASSAY PROCEDURE

  1. Collect a urine sample from test subject using a suitable clean container preferably glass.

  2. Refrigerated specimens or other materials should be equilibrated to room temperature before testing.

  3. Open the foil pouch at the notch, remove the test device, and label the device with specimen ID.

  4. Holding the dropper vertically as shown, add 4 drops of urine into the sample well "S" waiting 5 seconds between drops.

  5. Observe migration, or lateral flow of the sample across the entire test panel. Add additional sample drops if migration is not complete.

  6. Positive results may be observed as soon as 5 minutes, depending on the concentration of Barbiturates in the tested specimen. To confirm negative results, a complete reaction time of 8-10 minutes is required. Do not interpret results after 15 minutes.

INTERPRETATION OF TEST RESULTS:

Drug NewNeg

Drug New Pos

Drug New Inv

Negative

Positive

Invalid

The development of two lines, a control line and a test line, no matter how faint, in the test result window indicates a negative test screen for the targeted drug or drug metabolite.

The development of only one line, the control line, in the test result window indicates a positive test screen for the targeted drug or drug metabolite

The absence of a control line in the test result window indicates an invalid test result regardless of the presence of a test line in the test result window.

Positive One (1) pink-rose band appears in the control zone and no band appears in the test zone. A positive result indicates the Barbiturates level is 300 ng/ml or higher in the test urine sample.
Negative Two (2) pink-rose color bands appear, one in the control region and one in the test region. A negative result indicates that the Barbiturates level is below the detection sensitivity of 300 ng/ml. IMPORTANT: any line, no matter how faint, appearing in the test area confirms a negative test.
Invalid If there are no distinct color bands visible in both the test zone and the control zone or if there is a visible band in the test zone but not in the control zone, then the test is invalid. In this instance, re-testing of the specimen is recommended.

TEST AVAILABILITY

The QuickScreen™ One-Step Rapid Barbiturates test is now available to professionals and individuals concerned with Barbiturates abuse. The test is one-step, fast, self-contained, individually packaged and can be performed any time the targeted drug use or abuse is suspected. The tests come in order units of five (5) individual tests at a cost per single diagnosis/screen of $4.00 for the minimum order unit. For testing situations where sample adulteration is suspect, a Rapid Urine Adulteration test is available.

Test Description/Type Quantity Cost  

QuickScreen Barbiturates (BAR) Cassette Test

Five (5) Tests

$19.90

 

Fifteen (15) Tests

$45.00

 

Thirty (30) Tests

$75.00

 

One Hundred (100) Tests

$190.00

 

Two Hundred Fifty (250) Tests

$375.00

*If the order buttons are absent or inoperable, please use the pricing overview page to order.

URI Adult Vial

Urine Sample Validity
and Adulteration Test Strips are available:
Adulteration Test
 

Urine Sample Cup

Professional specimen collection containers available separately: Specimen Containers


 

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