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COCAINE (COC)
QuickScreen™ One-Step Rapid Drug Screen Test (Cassette type)

A Professional, Forensic Diagnostic Test for use in the Qualitative Detection of Cocaine and Cocaine Metabolite in Human Urine at the Standard Sensitivity Cut-off level of 300ng/ml Established as Standard by the U.S. Department of Health and Human Services, DOT, World Health Organization and SAMHSA.

INTENDED USE

For a comprehensive discussion on drug of abuse testing including typical detection time periods see the DRUG TESTING FAQ

The QuickScreen™ One-Step Rapid Cocaine Test is a rapid, visual, qualitative, competitive binding immunoassay for the detection of Cocaine (Erythyroxine Coca) metabolites (benzoylecgonine) in urine. The test provides a fast confirmation of the presence of Cocaine in the subject sample at a minimum cutoff sensitivity level of 300 ng/ml COC, the sensitivity level set by the National Institute on Drug Abuse. Note: Any positive test should be confirmed using quantitative methods by a qualified healthcare professional.
Note: This assay provides only a preliminary analytical test result. A more specific alternative chemical method such as high-pressure liquid chromatography (HPLC) or Gas chromatography/mass spectrometry (GC/MS) must be used in order to obtain a confirmed analytical result. For test situations requiring laboratory confirmation by such methods please refer to the QuickScreen Pro At Home Test Kit. Clinical consideration and professional judgment should be applied to all test results, particularly when preliminary positive results are indicated.

SUMMARY AND EXPLANATION OF THE TEST

Cocaine is an alkaloid present in Coca leaves (Erythyroxine coca). Its pharmacological properties, such as stimulation of the brain and central nervous system plus its profound euphoric effects, have been known for centuries. Cocaine has been used medicinally as a local anesthetic agent, but its addictive properties have minimized its modern daily use. Once considered a panacea for all sorts of ailments, Cocaine was readily available as the principle ingredient in patent medicines sold over the counter in the late 1800s and early 1900s. And indeed cocaine was the "secret" ingredient in the original recipe for Coca-Cola©.  Cocaine is most often self-administered by IV injection, nasal insufflations (snorting), and inhalation of vapor from the heating of the free base (smoking). The availability of “crack” (a street form of the free base) has increased cocaine use by the latter method.  Body elimination of cocaine is predominantly controlled by its bio-transformation. Very low concentrations of cocaine are detected in urine during the initial several hours, however benzoylecgonine, a hydrolytic degradation product or system metabolite of cocaine, persists in urine at a detectable level for 48 hours. Immunoassay testing has been developed for the determination of benzoylecgonine (cocaine metabolite) in urine at concentrations of 300 ng/ml or greater, the sensitivity cutoff level set by the National Institute on Drug Abuse. For comprehensive information on drug testing including typical half-life or drug detection periods see the Drug Testing FAQ.

The QuickScreen™ One-Step Rapid Cocaine Test utilizes a unique combination of monoclonal antibodies to selectively identify cocaine and its major metabolites in urine with a high degree of sensitivity. The test kit is a rapid, visually read screening method that does not require special instrumentation or training.

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The QuickScreen™ One-Step Rapid Cocaine Test technology incorporates a chromatographic absorbent device in which the drug or drug metabolites in the sample compete with a Cocaine derivative immobilized on a porous membrane for limited antibody sites. This is the preferred scientific method for qualitative assay. In the assay procedure, urine mixes with labeled antibody-dye conjugate and migrates through test device. When Cocaine levels are below 300 ng/ml (the detection cutoff sensitivity of the test) unbound antibody-dye conjugate binds to immobilized antigen conjugate in the Test Zone ("T"), producing a pink-rose colored band that indicates a negative result. Conversely, when Cocaine levels are above the detection limit, antibody-dye conjugate binds to the free drug, forming an antigen-antibody-dye complex. The complex competes with immobilized antigen conjugate in the Test Zone, preventing the development of a pink-rose colored band. Regardless of the test result, a color band is produced in the Control Zone ("C") by a non-specific sandwich dye conjugate reaction. This band serves as a built-in quality control by demonstrating antibody recognition, verifying that the reagents are chemically active, and confirming that the test was performed correctly.

MATERIALS PROVIDED:

Test Device and Dropper Pipette individually sealed in foil pouch with complete instructions.

ASSAY PROCEDURE

  1. Collect a urine sample from test subject using a suitable clean container preferably glass.

  2. Refrigerated specimens or other materials should be equilibrated to room temperature before testing.

  3. Open the foil pouch at the notch, remove the test device, and label the device with specimen ID.

  4. Holding the dropper vertically as shown, add 4 drops of urine into the sample well "S" waiting 5 seconds between drops.

  5. Observe migration, or lateral flow of the sample across the entire test panel. Add additional sample drops if migration is not complete.

  6. Positive results may be observed as soon as 5 minutes, depending on the concentration of Benzoylecgonine in the tested specimen. To confirm negative results, a complete reaction time of 8-10 minutes is required. Do not interpret results after 15 minutes.

INTERPRETATION OF TEST RESULTS:

Negative

Positive

Invalid

The development of two lines, a control line and a test line, in the test result window indicates a negative test screen for the targeted drug or drug metabolite.

The development of only one line, the control line, in the test result window indicates a positive test screen for the targeted drug or drug metabolite.

The absence of a control line in the test result window indicates an invalid test result regardless of the presence of a test line in the test result window.

Positive: One (1) pink-rose band appears in the control zone and no band appears in the test zone. A positive result indicates the Cocaine level is 300ng/ml or higher in the test urine sample.
Negative: One band appears in the test zone and one band appears in the control zone. A negative result indicates that the Cocaine level is below the detection sensitivity of 300ng/ml. IMPORTANT: any line, no matter how faint, appearing in the test area confirms a negative test.
Invalid If there are no distinct color bands visible in both the test zone and the control zone or if there is a visible band in the test zone but not in the control zone, then the test is invalid. In this instance, re-testing of the specimen is recommended.

TEST AVAILABILITY

The QuickScreen™ One-Step Rapid Cocaine Test is now available to professionals and individuals concerned with Cocaine abuse. The test is one-step, fast, self-contained, individually packaged and can be performed any time the targeted drug use or abuse is suspected. The tests come in order units of five (5) individual tests at a cost per single diagnosis/screen of $4.00 for the minimum order unit. For testing situations where sample adulteration is suspect, a Rapid Urine Adulteration test is available.

Test Description/Type

Quantity

Cost

 

QuickScreen Cocaine (COC) Cassette Test

Five (5) Tests

$19.90

 

Fifteen (15) Tests

$45.00

 

Thirty (30) Tests

$75.00

 

One Hundred (100) Tests

$190.00

 

Two Hundred Fifty (250) Tests

$375.00

*If the order buttons are absent or inoperable, please use the pricing overview page to order.

Urine Sample Validity
and Adulteration Test Strips are available:
Adulteration Test
 

Professional specimen collection containers available separately: Specimen Containers

 

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