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BARBITURATES (BAR)
QuickScreen™
One-Step Rapid Drug Screen Test (Cassette
type)

A
Professional, Forensic Diagnostic Test for use in the Qualitative Detection of Barbiturates
and their metabolites
in Human Urine at a Sensitivity
Level of 300 ng/ml Secobarbital Established as Standard by the U.S. Department of Health and Human
Services, DOT, World Health Organization and
SAMHSA.
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INTENDED
USE
For a comprehensive
discussion on drug of abuse testing including typical detection time periods
see the DRUG
TESTING FAQ |
The QuickScreen™ One-Step Rapid Barbiturates Test is a rapid, visual,
qualitative, competitive binding immunoassay for the detection of Barbiturates
in human urine. The test provides a fast confirmation of the
presence of Barbiturates in the subject sample at a minimum cutoff sensitivity
level of 300 ng/ml BAR, the sensitivity level set by the National Institute on
Drug Abuse and Departments of Health and Human Services. Note: Where indicated,
any positive test should be confirmed using quantitative methods by a qualified
testing laboratory.
Note:
This assay provides only a preliminary analytical test
result. A more specific alternative chemical method such as high-pressure liquid
chromatography (HPLC) or Gas chromatography/mass spectrometry (GC/MS) must be used in
order to obtain a confirmed analytical result. For test situations requiring laboratory
confirmation by such methods please refer to the QuickScreen Pro At Home Test Kit. Clinical consideration and professional judgment should be applied to all
test results, particularly when preliminary positive results are indicated.
SUMMARY
AND EXPLANATION OF THE TEST
Barbiturates
are a class of prescription drugs frequently abused. Barbiturates in normally prescribed
doses act in depressing the central nervous system while overdoses induce exhilaration,
sedation, and respiratory depression. Acute overdose results in respiratory
collapse, coma, and if not treated promptly, death. Classified generally as
depressants, barbiturates produce a state of intoxication that is remarkably
similar to alcohol intoxication. Symptoms include slurred speech, loss of motor
coordination and impaired judgment. Depending on the dose, frequency, and
duration of use, one can rapidly develop tolerance, physical dependence and
psychological dependence on barbiturates. Barbiturate abusers prefer the
short-acting and intermediate-acting barbiturates pentobarbital
(Nembutal), secobarbital (Seconal) and amobarbital (Amytal). Other
short-and intermediate-acting barbiturates are butalbital (Fiorinal, Fioricet),
butabarbital (Butisol), talbutal (Lotusate) and aprobarbital (Alurate). After
oral administration, the onset of drug effect is from 15 to 40 minutes and the
effects last up to 6 hours depending on dose administered and body type.
Teenagers often abuse barbiturates for the temporary "high" achieved
that simulates alcohol intoxication.
For comprehensive information on drug
testing including typical half-life or drug detection periods see the
Drug Testing FAQ.
The QuickScreen™ One-Step Rapid Barbiturates Test utilizes a unique combination of monoclonal antibodies to
selectively identify Barbiturates and their metabolites in urine with a high degree of
sensitivity. The test kit is a rapid, visually read screening method that does not require
special instrumentation or training.
PRINCIPLE
The QuickScreen™ One-Step Rapid Barbiturates Test technology incorporates a chromatographic absorbent device in which the drug or drug metabolites in the sample compete with a Barbiturates derivative immobilized on a porous membrane for limited antibody sites. This is the preferred scientific method for qualitative assay. In the assay procedure, urine mixes with labeled antibody-dye conjugate and migrates through test device. When Barbiturates levels are below 300 ng/ml (the detection cutoff sensitivity of the test) unbound antibody-dye conjugate binds to immobilized antigen conjugate in the Test Zone ("T"), producing a pink-rose colored band that indicates a negative result. Conversely, when Barbiturates levels are above the detection limit, antibody-dye conjugate binds to the free drug, forming an antigen-antibody-dye complex. The complex competes with immobilized antigen conjugate in the Test Zone, preventing the development of a pink-rose colored band. Regardless of the test result, a color band is produced in the Control Zone ("C") by a non-specific sandwich dye conjugate reaction. This band serves as a built-in quality control by demonstrating antibody recognition, verifying that the reagents are chemically active, and confirming that the test was performed correctly.
MATERIALS PROVIDED:
Test Device and Dropper Pipette
individually sealed in foil pouch with complete instructions.
ASSAY
PROCEDURE
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Collect a urine sample from test subject using a suitable clean container preferably glass.
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Refrigerated specimens or other materials should be equilibrated to room temperature before testing.
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Open the foil pouch at the notch, remove the test device, and label the device with specimen ID.
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Holding the dropper vertically as shown, add
4 drops of urine into the sample well "S"
waiting 5 seconds between drops.
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Observe migration, or lateral flow of the sample across the entire test
panel. Add additional sample drops if migration is not complete.
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Positive results may be observed as soon as
5 minutes, depending on the concentration of
Barbiturates in the tested specimen. To confirm negative results, a complete
reaction time of 8-10 minutes is required. Do not interpret results after
15 minutes.
INTERPRETATION
OF TEST RESULTS:
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Negative
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Positive
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Invalid
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The development of two lines, a control line and a test line,
no matter how faint, in the test result window indicates a negative test screen for the targeted drug or drug metabolite.
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The development of only one line, the control line, in the test result window indicates a positive test screen for the targeted drug or drug metabolite
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The absence of a control line in the test result window indicates an invalid test result regardless of the presence of a test line in the test result window.
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Positive One (1) pink-rose band appears in the control zone and no band appears in the test zone. A positive result indicates the Barbiturates level is 300 ng/ml or higher in the test urine sample.
Negative Two (2) pink-rose color bands appear, one in the control region and one in the test region. A negative result indicates that the Barbiturates level is below the detection sensitivity of 300 ng/ml. IMPORTANT: any line, no matter how faint, appearing in the test area confirms a negative test.
Invalid If there are no distinct color bands visible in both the test zone and the control zone or if there is a visible band in the test zone but not in the control zone, then the test is invalid. In this instance, re-testing of the specimen is recommended.
TEST AVAILABILITY
The QuickScreen™ One-Step Rapid Barbiturates test is now available to
professionals and individuals concerned with Barbiturates abuse.
The test is one-step, fast, self-contained, individually packaged and can be
performed any time the targeted drug use or abuse is suspected. The tests come
in order units of five (5) individual tests at a cost per single
diagnosis/screen of $4.00 for the minimum order unit. For testing situations
where sample adulteration is suspect, a Rapid
Urine Adulteration test is available.
Test Description/Type |
Quantity |
Cost |
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QuickScreen Barbiturates (BAR) Cassette Test |
Five
(5) Tests |
$19.90 |
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Fifteen
(15) Tests |
$45.00 |
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Thirty
(30) Tests |
$75.00 |
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One Hundred
(100) Tests |
$190.00 |
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Two Hundred
Fifty
(250) Tests |
$375.00 |
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*If the
order buttons are absent or inoperable, please use the
pricing overview page to order. |

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