Synthetic Cannabinoids (SPICE) One-Step Rapid Urine Drug Screen Test (Dip-scan type) This Professional, Forensic One-Step Synthetic Cannabinoids Drug Screen Test is a lateral flow immunoassay for the specific, qualitative detection of synthetic cannabinoid metabolites in human urine at a minimum cut-off level of 30ng/ml as Established as Standard by the U.S. Department of Health and Human Services, World Health Organization and SAMHSA. The synthetic cannabinoids detected by this test include, but are not limited to, the metabolites of JWH-018 and JWH-073. This assay is intended for forensic use only. This assay provides only a preliminary result.

INTENDED USE

The One-Step Synthetic Cannabinoids Spice Drug Screen Test is a lateral flow immunoassay for the specific, qualitative detection of synthetic cannabinoids metabolites in human urine at a cut-off level of 30 ng/ml. The synthetic cannabinoids detected by the test include, but are not limited to, the metabolites of JWH-018 and JWH-073. This assay is intended for forensic use only. This assay provides only a preliminary result. Careful consideration and professional judgment must be applied to any drug of abuse test result, particularly in evaluating a preliminary positive result. In order to obtain a confirmed analytical result, a more specific alternate chemical method is needed.
Note: This assay provides only a preliminary analytical test result. A more specific alternative chemical method such as high-pressure liquid chromatography (HPLC) or Gas chromatography/mass spectrometry (GC/MS) must be used in order to obtain a confirmed analytical result.  Clinical consideration and professional judgment should be applied to all test results, particularly when preliminary positive results are indicated. This assay is intended for forensic use only.

SUMMARY AND EXPLANATION OF THE TEST

Synthetic Cannabis (Spice) is a family of compounds that when consumed mimics the effects of Marijuana. It is also known by the brand names of K2 and Spice, both of which have largely become trademarks used to refer to any synthetic cannabinoids product. Studies suggest that synthetic cannabinoid intoxication is associated with acute psychosis, and the worsening of previously stable psychotic disorders among vulnerable individuals such as those with a family history of mental illness. JWH-018 and JWH-073 are the primary synthetic cannabinoid receptor agonists responsible for the euphoric and psychoactive effects that imitate Marijuana and are among the numerous compounds found in “herbal” incense or smoke blends. Most popular herbal smoking products are marketed under the brand names of K-2, K-3, Spice, Genie, Black Mombo, Pot-pouri, Buzz, Pulse, Hush, Mystery, Earthquake, Ocean Blue, Stinger, Yucatan Fire, as well as many others.

The excretion half-life of Synthetic Cannabinoids (Spice) metabolized in urine, estimated by different techniques, ranges from about two to ten days depending on a number of factors including frequency of use. The One-Step Synthetic Cannabinoids Drug Screen employs a unique combination of monoclonal antibodies to selectively identify JWH-018 and JWH-073 in urine with a high degree of sensitivity at 30 ng/ml, the established cut-off level for synthetic cannabinoids  The test device is a rapid, visually read screening test that does not require instrumentation and provides a fast indication of recent synthetic marijuana consumption. For comprehensive information on drug testing including typical half-life or drug detection periods see the Drug Testing FAQ.

PRINCIPLE

The One-Step Synthetic Cannabinoids (Spice) Drug Screen Test is based on the principle of competitive immunochemical reaction between a chemically labeled drug (drug-protein conjugate) and the drug or drug metabolites which may be present in the urine sample for the limited antibody binding sites. The test contains a nitrocellulose membrane strip pre-coated with drug-protein conjugate in the test region and a pad containing colored antibody-colloidal gold conjugate. During the test, the urine sample migrates upward and rehydrates the antibody-colloidal gold conjugate. The mixture then migrates along the membrane chromatographically by the capillary action to the immobilized drug-protein band on the test region. When drug is absent in the urine, the colored antibody-colloidal gold conjugate and immobilized drug-protein bind specifically to form a visible line in the test region. When drug is present in the urine, it will compete with drug-protein for the limited antibody sites. The line on the test region will become less intense with increasing drug concentration. When a sufficient concentration of drug is present in the urine, it will fill the limited antibody binding sites. This will prevent attachment of the colored antibody-colloidal gold conjugate to the drug-protein on the test region. Therefore, the presence of the line on the test region indicates a negative result for the drug and the absence of the test line on the test region indicates a preliminary positive result for the drug. A visible line generated by a different antigen/antibody reaction is also present at the control region of the test strip. This line should always appear, regardless of the presence of drugs or metabolites in the urine sample. This means that a negative urine sample will produce both test line and control line, and a positive urine sample will generate only control line. The presence of control line serves as a built-in control, which demonstrates that the test is performed properly.

MATERIALS PROVIDED:

One-Step Synthetic Cannabinoids Drug Screen Test individually sealed in foil pouch with complete instructions.

ASSAY PROCEDURE

1. Collect a urine sample from test subject using a suitable clean container preferably glass.

2. Refrigerated specimens or other materials should be equilibrated to room temperature before testing.

3. Open the foil pouch at the notch, remove the test device, and label the device with specimen ID. Remove test cap.

4. Holding the test vertically, dip the wick end into the sample urine. Do not dip past the line indicated on the top of the test wick

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5. Observe migration, or lateral flow of the sample across the entire test panel.

6. Positive results may be observed as soon as 2-3 minutes, depending on the concentration of Synthetic Cannabinoids in the tested specimen. Read results at 5 minutes. Do not interpret results after 60 minutes. If a permanent record is required, a scan or photo copy of the completed test device can be made.

INTERPRETATION OF TEST RESULTS:

Positive: One (1) pink-rose band appears in the control zone and no band appears in the test zone. A positive result indicates the presence of Synthetic Cannabinoids  (Spice) at a level of 30 ng/ml or higher in the test urine sample.
Negative: One band appears in the test zone and one band appears in the control zone. A negative result indicates the presence of Synthetic Cannabinoids (Spice) at a level of zero or less than 30 ng/ml in the test urine sample. IMPORTANT: any line, no matter how faint, appearing in the test area confirms a negative test.
Invalid: If there are no distinct color bands visible in both the test zone and the control zone or if there is a visible band in the test zone but not in the control zone, then the test is invalid. In this instance, re-testing of the specimen is recommended.

TEST AVAILABILITY

The One-Step Synthetic Cannabinoids (Spice) Drug Screen Test is available to professionals and individuals concerned with synthetic marijuana (Spice) abuse. The test is one-step, fast, self-contained, individually packaged and can be performed any time the targeted drug use or abuse is suspected. The tests come in order units of five (5), fifteen (15), thirty (30), one hundred (100) and two hundred fifty (250)  individual tests individually foil sealed in bulk. Each unit has a product insert and instruction sheet. For testing situations where urine sample adulteration is suspected, a Rapid Urine Adulteration test is available.

Test Description/Type	Quantity	Cost
Synthetic Cannabinoids (Spice) Urine Test Dip-Scan Type	Five (5) Tests	$37.50
	Fifteen (15) Tests	$97.50
	Thirty (30) Tests	$180.00
	One Hundred (100) Tests	$580.00
	Two Hundred Fifty (250) Tests	$1,250.00
*If the order buttons are absent or inoperable, please use the pricing overview page to order.

Urine Sample Validity and Adulteration Test Strips are available: Adulteration Test

Professional specimen collection containers available separately: Specimen Containers