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The test device, generally a cassette, contains membrane strips which are pre-coated with drug-protein conjugate (or antibody) on the test band. The colored antibodies (or drug-protein conjugate)-colloidal gold conjugate pads are placed at the end of the membrane. In the absence of drug in the urine, the solution of colored antibody (or drug-protein conjugate)-colloidal gold conjugate and urine moves upward chromatographically by the capillary action across the membrane. This solution then migrates to the immobilized drug-protein antibody (or drug-protein conjugate)-colloidal gold conjugate then attaches to the drug-protein conjugate (or antibody) to form a visible line as the antibody complexes with the drug conjugate. Therefore, the formation of a visible precipitant in the test zone occurs when the test urine is negative for the drug. When drug is present in the urine, the drug/metabolite antigen competes with drug-protein conjugate on the test band region for the limited antibody. When a sufficient concentration of drug is present, it will fill the limited antibody binding sites. This will prevent attachment of the colored antibody (or drug-protein conjugate)-colloidal gold conjugate to the drug-protein conjugate (or antibody) zone on the test band region. Therefore, absence of the color band on the test region indicates a positive result.
STORAGE REQUIREMENTS The test kit should be stored at refrigerated or at room temperature or 2-30° C (36-86° F) in the sealed pouch for the duration of the shelf-life. In general our test kits have a shelf-life exceeding 12 to 14 months. For details on how to use the QuickScreen Rapid drug tests, please refer to Drug Test Use
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