PHENCYCLIDINE (PCP) A.K.A. Angel Dust QuickScreen One-Step Rapid Drug Screen Test (Cassette type) A Professional, Forensic Diagnostic Test for use in the Qualitative Detection of Phencyclidine and its Metabolites (PCP)  in Human Urine at a minimum sensitivity cut-off level of 25 ng/ml Established as Standard by the U.S. Department of Health and Human Services, DOT, World Health Organization and SAMHSA.

INTENDED USE

The QuickScreen One-Step Rapid Phencyclidine Test is a rapid, qualitative, competitive binding immunoassay for the detection of Phencyclidine (PCP) metabolite in human urine. The test provides a fast confirmation of the presence of Phencyclidines in the subject sample at a minimum cutoff sensitivity level of 25ng/ml Phencyclidine. Note: Any positive test should be confirmed using quantitative methods by a qualified healthcare professional.
Note: This assay provides only a preliminary analytical test result. A more specific alternative chemical method such as high-pressure liquid chromatography (HPLC) or Gas chromatography/mass spectrometry (GC/MS) must be used in order to obtain a confirmed analytical result. For test situations requiring laboratory confirmation by such methods please refer to the QuickScreen Pro At Home Test Kit. Clinical consideration and professional judgment should be applied to all test results, particularly when preliminary positive results are indicated.

SUMMARY AND EXPLANATION OF THE TEST

Phencyclidine, commonly known as PCP or "angel dust" is used primarily as a recreational drug for its hallucinogenic effects. PCP is usually taken orally, by inhalation, insufflation or intravenously. It is well absorbed by the body following all routes of administration concentrating fastest in the fatty tissues and the brain. Unchanged PCP is excreted in the urine in moderate amounts (10% of the dose). The terminal half-life for PCP varies considerably with a range of 8-55 hours depending on quantity consumed, body size, metabolic rate and other factors unique to the user. Statistically, the average half-life is 18 hours. The effects of PCP, as with all hallucinogens, are unpredictable and variable. Symptoms include euphoria, extreme relaxation, increased strength, time and space distortions, anxiety, panic, hallucination and extreme erratic behavior. For comprehensive information on drug testing including typical half-life or drug detection periods see the Drug Testing FAQ.

For an initial immunoassay result, a cutoff value of 25ng/ml total Phencyclidine metabolites is required by the mandatory reference guidelines from the U.S. Department of Health and Human Services.⁸ All presumptive positive results should be confirmed at a cutoff concentration of 25 ng/ml Phencyclidine. The QuickScreen One-Step Phencyclidine Test employs a unique combination of monoclonal antibodies to selectively identify Phencyclidine and its derivative metabolites  in urine with a high degree of sensitivity. The test kit is a rapid, visually read screening test that does not require special instrumentation or technical training and provides a fast indication of recent Phencyclidine consumption.

PRINCIPLE

The QuickScreen One-Step Rapid Phencyclidine Test technology incorporates a chromatographic absorbent device in which the targeted drug or drug metabolites in the urine sample compete for limited antibody sites with an Phencyclidine control derivative immobilized on a porous membrane. This is the preferred scientific method for qualitative assay. In the assay procedure, urine mixes with a labeled antibody-dye conjugate and migrates through test device. When Phencyclidine levels are below 25 ng/ml (the detection cutoff sensitivity of the test) unbound antibody-dye conjugate binds to immobilized antigen conjugate in the Test Zone ("T"), producing a pink-rose colored band that indicates a negative result. Conversely, when Phencyclidine levels are above the detection limit, antibody-dye conjugate binds to the free drug, forming an antigen-antibody-dye complex. The complex competes with immobilized antigen conjugate in the Test Zone, preventing the development of a pink-rose colored band. Regardless of the test result, a color band is produced in the Control Zone ("C") by a non-specific sandwich dye conjugate reaction. This band serves as a built-in quality control by demonstrating antibody recognition, verifying that the reagents are chemically active, and confirming that the test was performed correctly.

MATERIALS PROVIDED:

Phencyclidine (PCP0 Test Device and Dropper Pipette individually sealed in foil pouch with complete instructions.

ASSAY PROCEDURE

1. Collect a urine sample from test subject using a suitable clean container preferably glass.

2. Refrigerated specimens or other materials should be equilibrated to room temperature before testing.

3. Open the foil pouch at the notch, remove the test device, and label the device with specimen ID.

4. Holding the dropper vertically as shown, add 4 drops of urine into the sample well "S" waiting 5 seconds between drops.

5. Observe migration, or lateral flow of the sample across the entire test panel. Add additional sample drops if migration is not complete.

6. Positive results may be observed as soon as 5 minutes, depending on the concentration of Phencyclidine in the tested specimen. To confirm negative results, a complete reaction time of 8-10 minutes is required. Do not interpret results after 15 minutes.

INTERPRETATION OF TEST RESULTS: 

Positive: One (1) pink-rose band appears in the control zone and no band appears in the test zone. A positive result indicates the PCP (Phencyclidine) level is 25 ng/ml or higher in the test urine sample.
Negative: One band appears in the test zone and one band appears in the control zone. A negative result indicates that the PCP (Phencyclidine) level is below the detection sensitivity of 25 ng/ml. IMPORTANT: any line, no matter how faint, appearing in the test area confirms a negative test.
Invalid: If there are no distinct color bands visible in both the test zone and the control zone or if there is a visible band in the test zone but not in the control zone, then the test is invalid. In this instance, re-testing of the specimen is recommended.

TEST AVAILABILITY

The QuickScreen One-Step Rapid Phencyclidine Test is now available to professionals and individuals concerned with Phencyclidine abuse. The test is one-step, fast, self-contained, individually packaged and can be performed any time the targeted drug use or abuse is suspected. The tests come in order units of five (5) individual tests at a cost per single diagnosis/screen of $4.00 for the minimum order unit. For testing situations where sample adulteration is suspect, a Rapid Urine Adulteration test is available.

Description	Sale Unit	Cost
QuickScreen Rapid Phencyclidine (PCP) Cassette Test	Five (5) Tests	$19.90
	Fifteen (15) Tests	$45.00
	Thirty (30) Tests	$75.00
	One Hundred (100) Tests	$190.00
	Two Hundred Fifty (250) Tests	$375.00
*If the order buttons are absent or inoperable, please use the pricing overview page to order.

Urine Sample Validity and Adulteration Test Strips are available: Adulteration Test

Professional specimen collection containers available separately: Specimen Containers