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Quality
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INTENDED USE
The QuickScreen™ One-Step Rapid Cocaine Test is a rapid, visual, qualitative, competitive binding immunoassay for the detection of Cocaine (Erythyroxine Coca) metabolites (benzoylecgonine) in urine. The test provides a fast confirmation of the presence of Cocaine in the subject sample at a minimum cutoff sensitivity level of 300 ng/ml COC, the sensitivity level set by the National Institute on Drug Abuse. Note: Any positive test should be confirmed using quantitative methods by a qualified healthcare professional. SUMMARY AND EXPLANATION OF THE TEST Cocaine is an alkaloid present in Coca leaves (Erythyroxine coca). Its pharmacological properties, such as stimulation of the brain and central nervous system plus its profound euphoric effects, have been known for centuries. Cocaine has been used medicinally as a local anesthetic agent, but its addictive properties have minimized its modern daily use. Once considered a panacea for all sorts of ailments, Cocaine was readily available as the principle ingredient in patent medicines sold over the counter in the late 1800s and early 1900s. And indeed cocaine was the "secret" ingredient in the original recipe for Coca-Cola©. Cocaine is most often self-administered by IV injection, nasal insufflations (snorting), and inhalation of vapor from the heating of the free base (smoking). The availability of “crack” (a street form of the free base) has increased cocaine use by the latter method. Body elimination of cocaine is predominantly controlled by its bio-transformation. Very low concentrations of cocaine are detected in urine during the initial several hours, however benzoylecgonine, a hydrolytic degradation product or system metabolite of cocaine, persists in urine at a detectable level for 48 hours. Immunoassay testing has been developed for the determination of benzoylecgonine (cocaine metabolite) in urine at concentrations of 300 ng/ml or greater, the sensitivity cutoff level set by the National Institute on Drug Abuse. For comprehensive information on drug testing including typical half-life or drug detection periods see the Drug Testing FAQ. The QuickScreen™ One-Step Rapid Cocaine Test utilizes a unique combination of monoclonal antibodies to
selectively identify cocaine and its major metabolites in urine with a high degree of
sensitivity. The test kit is a rapid, visually read screening method that does not require
special instrumentation or training.
PRINCIPLE
MATERIALS PROVIDED: Test Device and Dropper Pipette individually sealed in foil pouch with complete instructions. ASSAY PROCEDURE
INTERPRETATION OF TEST RESULTS:
Positive: One (1)
pink-rose band appears in the control zone and no band appears in the test zone.
A positive result indicates the Cocaine level is 300ng/ml or higher in the test
urine sample. The QuickScreen™ One-Step Rapid Cocaine Test is now available to professionals and individuals concerned with Cocaine abuse. The test is one-step, fast, self-contained, individually packaged and can be performed any time the targeted drug use or abuse is suspected. The tests come in order units of five (5) individual tests at a cost per single diagnosis/screen of $4.00 for the minimum order unit. For testing situations where sample adulteration is suspect, a Rapid Urine Adulteration test is available.
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The QuickScreen™ One-Step Rapid Cocaine Test technology incorporates a chromatographic absorbent device in which the drug or drug metabolites in the sample compete with a Cocaine derivative immobilized on a porous membrane for limited antibody sites. This is the preferred scientific method for qualitative assay. In the assay procedure, urine mixes with labeled antibody-dye conjugate and migrates through test device. When Cocaine levels are below 300 ng/ml (the detection cutoff sensitivity of the test) unbound antibody-dye conjugate binds to immobilized antigen conjugate in the Test Zone ("T"), producing a pink-rose colored band that indicates a negative result. Conversely, when Cocaine levels are above the detection limit, antibody-dye conjugate binds to the free drug, forming an antigen-antibody-dye complex. The complex competes with immobilized antigen conjugate in the Test Zone, preventing the development of a pink-rose colored band. Regardless of the test result, a color band is produced in the Control Zone ("C") by a non-specific sandwich dye conjugate reaction. This band serves as a built-in quality control by demonstrating antibody recognition, verifying that the reagents are chemically active, and confirming that the test was performed correctly.
