MARIJUANA (THC) QuickScreen™ One-Step Rapid Drug Screen Test (Cassette type) A Professional, Forensic Diagnostic Test for use in the Qualitative Detection of Marijuana (THC metabolite) in Human Urine at a minimum sensitivity cut-off level of 50 ng/ml Established as Standard by the U.S. Department of Health and Human Services, World Health Organization and SAMHSA.

INTENDED USE

The QuickScreen™ One-Step Rapid Marijuana Test is a rapid, qualitative, competitive binding immunoassay for the detection of Tetrahydrocannibinol (THC) metabolites in urine. The test provides a fast (8 minute) confirmation of the presence of THC in the subject sample at a minimum cutoff sensitivity level of 50ng/ml THC. Note: it is recommended that any positive test be confirmed using quantitative methods by a qualified healthcare professional.
Note: This assay provides only a preliminary analytical test result. A more specific alternative chemical method such as high-pressure liquid chromatography (HPLC) or Gas chromatography/mass spectrometry (GC/MS) must be used in order to obtain a confirmed analytical result. For test situations requiring laboratory confirmation by such methods please refer to the QuickScreen Pro At Home Test Kit. Clinical consideration and professional judgment should be applied to all test results, particularly when preliminary positive results are indicated.

SUMMARY AND EXPLANATION OF THE TEST

Marijuana, a hallucinogen, is commonly ingested by smoking, but may also be orally ingested. Marijuana is most commonly the drug of choice among teenagers and young adults. The hallucinogenic effect of Marijuana can lead to irrational behavior, disorientation, and paranoia. After smoking or oral administration, the major psychoactive compound, Tetrahydrocannibinol, is extensively metabolized before excretion. Tetrahydrocannabinol (THC) is generally accepted to be the principle active component in marijuana, although other cannabinoids are likely to contribute to the physiological activity of marijuana. Tetrahydrocannabinol is rapidly absorbed by inhalation and through the gastrointestinal tract. It is almost completely metabolized. The predominant metabolite 11-Nor-Δ⁹-THC-9-carboxylic Acid (or THCA), is found in the plasma and urine along with other compounds.  Very low concentrations of THC may be detected in urine during the initial several hours, but THCA persists in urine at a detectable concentration for many days after smoking.

The excretion half-life of Cannabinoids metabolized in urine, estimated by different techniques, ranges from two to ten days. The QuickScreen™ One-Step Marijuana Test employs a unique combination of monoclonal antibodies to selectively identify THC metabolites in urine with a high degree of sensitivity. The test device is a rapid, visually read screening test that does not require instrumentation and provides a fast indication of recent marijuana consumption. For comprehensive information on drug testing including typical half-life or drug detection periods see the Drug Testing FAQ.

PRINCIPLE

The QuickScreen™ One-Step Rapid Marijuana Test technology incorporates a chromatographic absorbent device in which the drug or drug metabolites in the sample compete with a THC derivative immobilized on a porous membrane for limited antibody sites. This is the preferred scientific method for qualitative assay. In the assay procedure, urine mixes with labeled antibody-dye conjugate and migrates through test device. When THC levels are below 50 ng/ml (the detection cutoff sensitivity of the test) unbound antibody-dye conjugate binds to immobilized antigen conjugate in the Test Zone ("T"), producing a pink-rose colored band that indicates a negative result. Conversely, when THC levels are above the detection limit, antibody-dye conjugate binds to the free drug, forming an antigen-antibody-dye complex. The complex competes with immobilized antigen conjugate in the Test Zone, preventing the development of a pink-rose colored band. Regardless of the test result, a color band is produced in the Control Zone ("C") by a non-specific sandwich dye conjugate reaction. This band serves as a built-in quality control by demonstrating antibody recognition, verifying that the reagents are chemically active, and confirming that the test was performed correctly.

MATERIALS PROVIDED:

QuickScreen™ One-Step THC test device and dropper pipette individually sealed in foil pouch with complete instructions.

ASSAY PROCEDURE

1. Collect a urine sample from test subject using a suitable clean container preferably glass.

2. Refrigerated specimens or other materials should be equilibrated to room temperature before testing.

3. Open the foil pouch at the notch, remove the test device, and label the device with specimen ID.

4. Holding the dropper vertically as shown, add 4 drops of urine into the sample well "S" waiting 5 seconds between drops.

5. Observe migration, or lateral flow of the sample across the entire test panel. Add additional sample drops if migration is not complete.

6. Positive results may be observed as soon as 5 minutes, depending on the concentration of the Marijuana in the tested specimen. To confirm negative results, a complete reaction time of 8-10 minutes is required. Do not interpret results after 15 minutes.

INTERPRETATION OF TEST RESULTS:

Positive: One (1) pink-rose band appears in the control zone and no band appears in the test zone. A positive result indicates the THC (Marijuana) level is 50 ng/ml or higher in the test urine sample.
Negative: One band appears in the test zone and one band appears in the control zone. A negative result indicates that the THC (Marijuana) level is below the detection sensitivity of 50 ng/ml. IMPORTANT: any line, no matter how faint, appearing in the test area confirms a negative test.
Invalid: If there are no distinct color bands visible in both the test zone and the control zone or if there is a visible band in the test zone but not in the control zone, then the test is invalid. In this instance, re-testing of the specimen is recommended.

TEST AVAILABILITY

The QuickScreen™ One-Step Rapid Marijuana Test is now available to professionals and individuals concerned with marijuana abuse. The test is one-step, fast, self-contained, individually packaged and can be performed any time the targeted drug use or abuse is suspected. The tests come in order units of five (5) individual tests at a cost per single diagnosis/screen of $4.00 for the minimum order unit. For testing situations where sample adulteration is suspect, a Rapid Urine Adulteration test is available.

Test Description/Type	Quantity	Cost
QuickScreen Marijuana (THC) Cassette Test	Five (5) Tests	$19.90
	Fifteen (15) Tests	$45.00
	Thirty (30) Tests	$75.00
	One Hundred (100) Tests	$190.00
	Two Hundred Fifty (250) Tests	$375.00
*If the order buttons are absent or inoperable, please use the pricing overview page to order.

Urine Sample Validity and Adulteration Test Strips are available: Adulteration Test

Professional specimen collection containers available separately: Specimen Containers